Search results for " multifunctional T cells"

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Optimized Protocol for the Detection of Multifunctional Epitope-Specific CD4+ T Cells Combining MHC-II Tetramer and Intracellular Cytokine Staining T…

2019

Analysis of multifunctional CD4+ T cells is fundamental for characterizing the immune responses to vaccination or infection. Major histocompatibility complex (MHC)/peptide tetramers represent a powerful technology for the detection of antigen-specific T cells by specific binding to their T-cell receptor, and their combination with functional assays is fundamental for characterizing the antigen-specific immune response. Here we optimized a protocol for the detection of multiple intracellular cytokines within epitope-specific CD4+ T cells identified by the MHC class II tetramer technology. The optimal procedure for assessing the functional activity of tetramer-binding CD4+ T cells was based o…

CD4-Positive T-Lymphocyteslcsh:Immunologic diseases. Allergymedicine.medical_treatmentImmunologyEpitopes T-LymphocyteMajor histocompatibility complexEpitopeimmune responseMice03 medical and health sciences0302 clinical medicineImmune systemMHC-II tetramersTetramerMethodsmedicineAnimalsImmunology and Allergy030304 developmental biology0303 health sciencesMHC class IIMHC-II tetramers ICS cytokines multifunctional T cells flow cytometry immune response vaccinationStaining and LabelingbiologyChemistryflow cytometryHistocompatibility Antigens Class IIvaccinationmultifunctional T cellscytokines3. Good healthCell biologyCytokineICSbiology.proteinFemaleAntibodyPeptideslcsh:RC581-607Intracellular030215 immunologyFrontiers in Immunology
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